Dic microscopy is a technique which uses gradients in the optical path length and phase shifts to make phase objects visible under the light microscope.
Dic transmitted light microscopy.
When compared to the typical configuration employed in transmitted light microscopy the critical instrument parameters for reflected or episcopic light differential interference contrast dic are much simpler primarily because only a single birefringent nomarski or wollaston prism is required and the objective serves as both the condenser and image forming optical system.
In this way it is possible to observe living cells and organisms with adequate contrast and resolution.
Differential interference contrast.
Differential interference contrast reflected light dic microscopy.
Directly transmitted light which did not.
Differential interference contrast dic microscopy also known as nomarski interference contrast nic or nomarski microscopy is an optical microscopy technique used to enhance the contrast in unstained transparent samples dic works on the principle of interferometry to gain information about the optical path length of the sample to see otherwise invisible features.
When compared to the typical configuration employed in transmitted light microscopy the critical instrument parameters for reflected or episcopic light differential interference contrast dic are much simpler primarily because only a single birefringent nomarski or wollaston prism is required and the objective serves as.